PCR Techniques Course
Gain mastery in PCR and qPCR techniques from sample receipt through to result reporting. This course covers assay design, nucleic acid extraction, contamination control, plate layouts, and data interpretation, enabling you to produce reliable, audit-ready results in high-throughput lab settings. Participants will develop hands-on skills for consistent, defensible outcomes in molecular diagnostics.

4 to 360h flexible workload
certificate valid in your country
What will I learn?
This course delivers hands-on, step-by-step instruction in PCR and qPCR assay design, nucleic acid extraction methods, contamination prevention strategies, and thermal cycling protocols. You will learn to select and validate established protocols, create effective plate layouts, utilize and analyze controls, resolve weak or ambiguous results, and follow rigorous quality management and documentation standards to ensure trustworthy, reproducible data in laboratory environments.
Elevify advantages
Develop skills
- Design diagnostic PCR/qPCR assays by selecting targets, primers, and probes quickly.
- Execute clean nucleic acid extraction workflows to avoid contamination and preserve RNA integrity.
- Set up and optimize qPCR runs including master mixes, thermal profiles, and plate layouts.
- Interpret PCR/qPCR data with accurate Ct values, weak positives, controls, and QC metrics.
- Implement laboratory quality systems with records, SOPs, biosafety, and result documentation.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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