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PCR Techniques Course

4.5/5
PCR Techniques Course
4.5 in rating
4 to 360h flexible workload
certificate valid in your country

What will I learn?

This course delivers hands-on, step-by-step instruction in PCR and qPCR assay design, nucleic acid extraction methods, contamination prevention strategies, and thermal cycling protocols. You will learn to select and validate established protocols, create effective plate layouts, utilize and analyze controls, resolve weak or ambiguous results, and follow rigorous quality management and documentation standards to ensure trustworthy, reproducible data in laboratory environments.

Elevify advantages

Develop skills

  • Design diagnostic PCR/qPCR assays by selecting targets, primers, and probes quickly.
  • Execute clean nucleic acid extraction workflows to avoid contamination and preserve RNA integrity.
  • Set up and optimize qPCR runs including master mixes, thermal profiles, and plate layouts.
  • Interpret PCR/qPCR data with accurate Ct values, weak positives, controls, and QC metrics.
  • Implement laboratory quality systems with records, SOPs, biosafety, and result documentation.

Suggested summary

Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.
Workload: between 4 and 360 hours

What our students are saying

Your classes are perfect. I purchased the one-year package and finally have the opportunity to follow various topics of interest without needing to switch platforms... I thank you for everything you do, I've already recommended you to other people...
Giulio Carlo
Giulio CarloDigital Marketing Student
I like how the lessons are straight to the point and how I can change chapters and skip content I don't need.
Mariana Ferres
Mariana FerresPhotography Student
I like the content and the presentation style and video transcription, which speeds up the process!
Luciana Alvarenga
Luciana AlvarengaNail Design Student
The platform is fast, simple to use. The diversity of content and complementary videos really help with learning.
André Felipe
André FelipePrompt Engineering Student

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