Electrophoresis Course
Gain expertise in electrophoresis techniques, from foundational concepts to producing high-quality, publication-ready results. The course covers agarose gels, SDS-PAGE, western blotting, PCR and RFLP design, troubleshooting, quantitative protein analysis, and proper documentation, enabling confident performance in molecular biology labs anywhere.

4 to 360h flexible workload
certificate valid in your country
What will I learn?
This course equips you with hands-on skills to design and execute agarose gels, SDS-PAGE, and western blots effectively. You'll grasp essential electrophoresis principles, select appropriate gels and buffers, design PCR products, apply RFLP methods, and master optimization, troubleshooting, quantitative analysis, and documentation for reliable, reproducible DNA and protein data ready for publication.
Elevify advantages
Develop skills
- Plan agarose and SDS-PAGE experiments efficiently by selecting optimal gel percentages, buffers, ladders, and voltages.
- Design effective PCR and RFLP assays, choosing suitable primers, amplicon sizes, and markers for distinct bands.
- Perform superior western blots through optimized transfer, blocking, antibody use, and detection methods.
- Quickly troubleshoot issues in gels and blots, resolving problems like smearing, faint bands, or high background.
- Document and archive experiments thoroughly, including file naming, storage of gels and blots, and metadata tracking.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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