CRISPR Course
This course equips learners with practical skills to design, execute, and validate CRISPR-based single-base edits for treating monogenic blood disorders using hematopoietic stem cells (HSCs), covering technical, analytical, and ethical aspects.

4 to 360h flexible workload
certificate valid in your country
What will I learn?
This CRISPR Course provides a focused, practical roadmap to design and optimize precise single-base edits for monogenic blood disorders. You will learn guide RNA selection, repair template design, base and prime editing choices, ex vivo HSC workflows, and delivery options, plus robust molecular and functional assays, off-target and genotoxicity analysis, and essential safety, ethical, and regulatory considerations.
Elevify advantages
Develop skills
- Design CRISPR guides: select PAMs, score on-targets, and limit bystander edits.
- Engineer precise single-base fixes: choose HDR, base editing, or prime editing.
- Optimize HSC ex vivo editing: RNP delivery, culture conditions, and scale-up.
- Validate edits rigorously: NGS, allele-specific assays, and functional readouts.
- Control off-targets and ethics: safety assays, risk review, and compliance.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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