Transgene Course
Gain expertise in transgene design for tomato fungal resistance. Cover construct design, transformation processes, molecular checks, biosafety measures, and regulatory essentials to develop safe, effective disease-resistant tomato varieties for biological research in Botswana contexts.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This Transgene Course offers a practical guide to designing, transforming, and validating tomato lines resistant to fungal diseases. Key topics include transgene construct design, promoter selection, RNAi and HIGS methods, transformation and regeneration techniques, molecular screening, phenotyping protocols, plus biosafety, risk assessment, and regulatory documentation for compliant outcomes.
Elevify advantages
Develop skills
- Design transgene constructs with optimal promoters, markers, and protein targeting.
- Perform tomato transformation using Agrobacterium methods and quick plant regeneration.
- Confirm transgene expression via PCR, qPCR, RT-qPCR, Western, ELISA, and reporters.
- Set up disease assays with inoculation techniques, phenotyping measures, and data review.
- Follow biosafety rules and prepare regulatory documents through risk evaluation.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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