Restriction Enzymes Course
Gain confidence in using restriction enzymes for cloning in biological sciences. This course teaches primer design, vector selection, digest planning, ligation optimisation, transformation, and colony screening to build and verify expression constructs reliably in your lab projects.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course offers a practical guide from primer design to verified constructs using restriction enzymes. You will learn to create primers ready for restriction, select expression vectors, execute precise digests, optimise ligations, perform colony PCR and minipreps, conduct diagnostic digests, troubleshoot issues, and explore other cloning methods for consistent results in your lab work.
Elevify advantages
Develop skills
- Design restriction cloning with precise enzyme sites for clean directional inserts.
- Master PCR primers that are Tm-balanced, frame-safe, and include restriction sites.
- Select optimal plasmids, tags, and promoters for efficient expression.
- Perform efficient digests, ligations, and E. coli transformations.
- Verify clones using digests, colony PCR, and sequencing.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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