Proteomics Course
This course equips you with comprehensive skills in cell-line proteomics, covering everything from sample preparation to actionable biological insights. You will learn LC-MS/MS instrument setup, DDA and DIA acquisition methods, experimental design principles, quality control measures, bioinformatics processing, and statistical analysis techniques to identify robust protein signatures and validate key pathways in inflammation and disease studies.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
Gain a complete practical guide to designing and running reliable LC-MS/MS proteomics experiments. Master high-resolution mass spectrometry setup, DDA and DIA optimisation, cell-line study planning, and quantitative method selection. Develop essential bioinformatics, statistical analysis, functional interpretation, and validation skills for producing trustworthy, publication-ready protein data.
Elevify advantages
Develop skills
- Design efficient LC-MS/MS proteomics workflows by selecting optimal methods, quantification, and enrichment strategies swiftly.
- Set up and optimise Orbitrap or timsTOF instruments for DDA and DIA runs to ensure high-quality data output.
- Process raw mass spectrometry files through database searching, FDR control, and reliable quantification pipelines.
- Perform statistical analysis on proteomics data to identify differential proteins and map biological pathways effectively.
- Validate key findings using PRM/SRM assays, Western blots, and strategies to handle batch effects and missing values.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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