Electrophoresis Course
Gain expertise in electrophoresis techniques, from foundational concepts to producing reliable, quantitative data ready for publication. The course covers agarose gels, SDS-PAGE, western blotting, PCR and RFLP design, troubleshooting, and proper documentation, ideal for molecular biology labs seeking consistent results.

from 4 to 360h flexible workload
valid certificate in your country
What will I learn?
This course equips you with hands-on skills to set up and perform agarose gels, SDS-PAGE, and western blots effectively. You'll grasp essential electrophoresis principles, select gels and buffers, design PCR products, apply RFLP methods, optimise runs, troubleshoot issues, analyse quantitative protein data, and document findings for reproducible, publishable DNA and protein outcomes.
Elevify advantages
Develop skills
- Plan agarose and SDS-PAGE gels efficiently by selecting appropriate percentages, buffers, ladders, and voltages.
- Design effective PCR and RFLP assays, choosing primers, amplicon sizes, and markers for distinct bands.
- Execute superior western blots through optimised transfer, blocking, antibody use, and detection methods.
- Quickly troubleshoot gel and blot problems like smearing, faint bands, or high background.
- Document and store experiments properly, including file naming, gel preservation, and metadata tracking.
Suggested summary
Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.What our students say
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