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Restriction Enzymes Course

Restriction Enzymes Course
4 to 360 hours flexible workload
valid certificate in your country

What will I learn?

This course offers a streamlined practical guide from primer design to confirmed constructs using restriction enzymes. You'll learn to create primers ready for restriction, select expression vectors, plan accurate digests, optimise ligations, master colony PCR, minipreps and diagnostic digests, plus troubleshoot issues and alternative cloning techniques for dependable results in your lab work.

Elevify advantages

Develop skills

  • Design restriction cloning with precise enzyme sites for clean directional inserts.
  • Master PCR primers that are Tm-balanced, frame-safe and include restriction sites.
  • Select optimal plasmids, tags and promoters for rapid expression.
  • Perform efficient digests, ligations and E. coli transformations.
  • Verify clones using digests, colony PCR and sequencing.

Suggested summary

Before starting, you can change the chapters and the workload. Choose which chapter to start with. Add or remove chapters. Increase or decrease the course workload.
Workload: between 4 and 360 hours

What our students say

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EmersonPolice Investigator
The course was essential to meet the expectations of my boss and the company I work for.
SilviaNurse
Great course. Lots of valuable information.
WiltonCivil Firefighter

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